Princeton SFC 世界领导者
FLM Comix 超神色谱柱
FLM Chiral 手性色谱柱
FLM F-BronX 气相GC色谱柱-毛细管色谱柱
FLM TitanK 有机色谱柱
Sielc Promix 生物色谱柱App ID: FLM-C210057
果汁中的維生素C在Comixsil HAX混合型柱上的定量分析
Equipment
Agilent 1100 HPLC system with DAD Analytical Balance
HPLC Column: Comixsil HAX 3 um 3.0x100 mm(P/N: FMD-HAX3-YONU)
Materials:
Fruit juices
ACN (Tedia, catalog #AS1127)
Distilled water (Aldrich, catalog #270733) Ammonium acetate (Aldrich, catalog #7262) Acetic acid (Aldrich, catalog #6283)
Vitamin C (Aldrich, catalog #A92902)
Method Description 2
HPLC Pump set up: A: DI water, B: Acetonitrile, C: 100 mM Ammonium acetate pH 4
Mobile phase composition: : A: 30%, B: 60%, C: 10%
Vitamin C Calibration and Sample Analysis
|
Concentration, mg/ml |
Peak Area Average, mAU*s |
Peak Area, Inj. 1, mAU*s |
Peak Area, Inj. 2, mAU*s |
Peak Area, Inj. 3, mAU*s |
Concentration of Vitamin C, mg/ml |
|
0.02 |
548 |
542 |
555 |
547 |
- |
|
0.05 |
1352 |
1359 |
1315 |
1382 |
- |
|
0.1 |
2759 |
2750 |
2811 |
2717 |
- |
|
0.2 |
6149 |
6143 |
6156 |
6148 |
- |
|
0.5 |
13809 |
13875 |
13783 |
13768 |
- |
|
White Grape MP |
13792 |
13813 |
13888 |
13675 |
0.52 |
|
Apple Mott's |
20886 |
20919 |
20884 |
20855 |
0.79 |
|
Cranberry OS |
19377 |
19462 |
19348 |
19321 |
0.73 |
|
Cranberry MP |
1205 |
1197 |
1208 |
1211 |
0.03 |
|
Lemon Motts |
6691 |
6709 |
6678 |
6686 |
0.24 |
|
Lime Motts |
6756 |
6773 |
6739 |
6756 |
0.25 |
